Journal: Cancer Science
Article Title: Protein interacting with C α kinase 1 (PICK1) is involved in promoting tumor growth and correlates with poor prognosis of human breast cancer
doi: 10.1111/j.1349-7006.2010.01566.x
Figure Lengend Snippet: The prognostic significance of high protein interacting with C α kinase 1 (PICK1) protein expression in the entire patient population. The graphs show the effect of PICK1 expression within cancer cells (a) on patient overall survival (log‐rank test) and stratified analysis by lymph nodes status (b), Her‐2/neu status (c), hormone receptor (HR) status (d), and molecular subclassification (e–h). High PICK1 expression in cancer cells (a) associated with decreased overall survival (OS) (P < 0.0001, respectively). When patients’ OS were stratified by lymph node status, Her‐2/neu status, hormone receptor status, and molecular subclassification status, PICK1 expression was associated with reduced OS in the Her‐2/neu‐positive and ‐negative (c, P = 0.019 and P = 0.003, respectively), HR‐positive and ‐negative (d, P = 0.007 and P < 0.0001, respectively), and lymph node ‐positive subgroups (b, P < 0.0001). Molecular subclassification: HR−/HER‐2+ and HR−/HER‐2− (g–f, P = 0.0.041 and P = 0.002, respectively).
Article Snippet: The antibodies and the dilution factors were as follows: polyclonal antibody against PICK1 (1:75) from ProteinTech Group (Chicago, IL, USA); Dako Clone ID5 antibody against ER (1:450) (Carpinteria, CA, USA); Dako Clone IA6 antibody against PR (1:200); and Dako polyclonal antibody against Her‐2/neu (1:1000).
Techniques: Expressing